Fig. 4.

TMEM132A regulates integrin protein levels. (A) Integrin proteins α1, α2 and β1, which form the collagen binding receptors, were repressed by TMEM132A knockdown in HeLa cells (48 h post-transfection; GAPDH internal control). (B) Quantification of β1, α1 and α2 integrin protein levels from western blot band intensity (n=3). Data are mean±s.d. *P<0.05, **P<0.01 (two-tailed Student's t-test). (C) Representative integrin heterodimers that bind different matrix proteins. Collagen-binding integrin heterodimers that are repressed by TMEM132A loss are highlighted in green. (D) TMEM132A knockdown shows decreased phosphorylation of FAK and SRC in HeLa cells 48 h post-transfection. Relative band intensity of phosphorylated proteins normalized to corresponding total proteins is given below corresponding panel. Relative protein levels <0.80 are shown in red.