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. 2022 Aug 29;119(37):e2201137119. doi: 10.1073/pnas.2201137119

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Copyright © 2022 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).

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Fig. 2. — NewStar2 administration decreases Aβ plaque load and BACE-1 expression levels. (A) Plaque load after PBS or NewStar2 administration via IP injections. (B) Plaque load after PBS or NewStar2 administration via osmotic pumps. Representative images of hippocampus are shown. Scale bar, 100 µm. (C–E) Quantification of Aβ plaque coverage in hippocampus (IP injections: PBS, n = 12, NewStar2, n = 11; osmotic pumps: PBS, n = 8, NewStar2, n = 9; two-way ANOVA, Bonferroni post hoc analysis: F_1,36 = 6.68, degrees of freedom [DF] = 1) (C); corpus callosum (IP injections: PBS, n = 12, NewStar2, n = 11; osmotic pumps: PBS, n = 8, NewStar2, n = 9; two-way ANOVA, Bonferroni post hoc analysis: F_1,36 = 5.58, DF = 1) (D); and cortex (IP injections: PBS, n = 12, NewStar2, n = 11; osmotic pumps (PBS: n = 8, NewStar2, n = 9; two-way ANOVA, Bonferroni post hoc analysis: F_1,36 = 4.23, DF = 1) (E). (F) Sections from tissues of mice treated with PBS compared with those of mice treated with NewStar2 administered via IP injections or osmotic pumps. Sections were stained with 6e10 (magenta) for Aβ plaques and anti–BACE-1 (green) for BACE-1. Representative images of hippocampus are shown. Scale bar, 50 µm. (G–I) Quantification of BACE-1 coverage in hippocampus (IP injections: PBS, n = 12, NewStar2, n = 11; osmotic pumps: PBS, n = 8, NewStar2, n = 9; two-way ANOVA, Bonferroni post hoc analysis: F_1,36 = 9.64, DF = 1) (G); corpus callosum (IP injections: PBS, n = 12, NewStar2, n = 11; osmotic pumps: PBS, n = 8; NewStar2, n = 9; two-way ANOVA, Bonferroni post hoc analysis: F_1,36 = 14.93, DF = 1) (H); and cortex (IP injections: PBS, n = 12, NewStar2, n = 11; osmotic pumps (PBS, n = 8, NewStar2, n = 9; two-way ANOVA, Bonferroni post hoc analysis: F_1,36 = 2.09, DF = 1) (I). Data are presented as mean ± SEM. *P < 0.05; ***P < 0.001.