Fig. 4.
NewStar2 stimulates microglial phagocytosis and Aβ plaque uptake. (A) Sections from tissues of mice treated with PBS or NewStar2 via IP injections or osmotic pumps. Sections were stained with 6e10 (magenta) for Aβ plaques and CD68 (green) for phagocytic microglia. Representative images of the hippocampus area are shown. Scale bar, 25 µm. (B) Quantification of CD68-positive microglia around the border of Aβ plaques at a distance of 15 µm, shown in A (IP injections: PBS, n = 12 mice and n = 43 plaques, NewStar2, n = 11 mice and n = 29 plaques; osmotic pumps: PBS, n = 8 mice and n = 32 plaques, NewStar2, n = 9 and n = 24 plaques; two-way ANOVA, Bonferroni post hoc analysis: F1,36 = 11.35, degrees of freedom = 1). Data are presented as mean ± SEM. *P < 0.05; **P < 0.01.