FIG. 1.

2-D gel electrophoresis patterns of proteins isolated from cells of P. aeruginosa strain PAO1 (top) and its isogenic mucA22 derivative PDO300 (bottom). Cells were grown in L broth with aeration and collected in late logarithmic phase of growth, and total proteins were extracted for a 2-D gel analysis. The gels were stained with Coomassie blue. The arrowhead points to the internal standard, tropomyosin (32.7 kDa, pI 5.2). The molecular mass standard lines are from myosin (220 kDa), phosphorylase A (94 kDa), catalase (60 kDa), actin (43 kDa), carbonic anhydrase (29 kDa), and lysozyme (14 kDa). The spots that appeared more intense in one strain than in the other were marked with arrows and given the designations described in Table 2. Gels were trans-blotted onto polyvinylidene difluoride membranes, and spots were subjected to an amino-terminal sequence analysis for identification (Table 2).