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. 2022 Sep 17;8:387. doi: 10.1038/s41420-022-01185-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Representative confocal images showing expression of CNGA1 (red) and CNGB1 protein (green) in retinal cross sections of wild-type (1 month postnatal (PM1); A, D), and Cnga1^Y509C/Y509C mouse retinas (B, C, E, F) at PM1 and PM6. Cnga1^Y509C/Y509C mice are lacking CNGA1 and CNGB1 already at PM1. Cell nuclei were stained with DAPI (blue). OS, outer segments; IS, inner segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer. Scale bar marks 20 µm. G–I Western blot analysis of Cnga1^Y509C/Y509C mouse retinas at PM1 and PM6 using CNGA1- and CNGB1-antibodies. β-Actin was used as control. Western blot staining (G) and quantification of CNGA1 (H) and CNGB1 (I) expression confirm the findings of immunohistochemistry. RT-qPCR of Cnga1^Y509C/Y509C mouse retinas at PM1 and PM6 with Cnga1- (J) and Cngb1-specific primers (K). Mutant mice at PM1 still express Cnga1 and Cngb1 transcript. This expression is reduced at PM6. N = 3 biological and technical replicates. Values are given as mean ± SEM (one-way ANOVA paired with Tukey’s post-hoc test; **p ≤ 0.01, ***p ≤ 0.001).