FIG. 3.

SDS-PAGE analysis of MBP-HyuA and AllB. Aliquots (5 μl each) of the protein samples were analyzed on a 10% polyacrylamide gel under denaturing conditions. (A) Analysis of the MBP-HyuA fusion protein. Lane 1, whole-cell extracts after induction for 2 h at 37°C; lane 2, purified MBP-HyuA fusion protein (95 kDa). The arrow indicates the MBP-HyuA fusion protein. (B) Analysis of purified AllB. Lane 1, whole-cell extract; lane 2, fractions eluted from the ion-exchange chromatography column; lane 3, purified AllB from the active fractions after gel filtration column chromatography. The arrow indicates AllB.