Table 1.
Inter-assay precision and accuracy for quality control (QC) of plasma samples spiked with antidepressant drugs. AMI, amitriptyline; MAP, maprotiline; VEN, venlafaxine; NOR, nortriptyline; IMI, imipramine; DES, desipramine for different methods applied. LLOQ lower limit of quantification, LQC low QC, MQC medium QC, HQC high QC
| QC level | Method | AMI | MAP | VEN | NOR | IMI | DES | |
|---|---|---|---|---|---|---|---|---|
|
LLOQ (25 ng/mL) |
LC-vDMS-MS (SIM) | Accuracy (%) | 102 | 91.8 | 104 | 100 | 107 | 97.3 |
| Precision (%) | 10.4 | 8.75 | 12.9 | 8.91 | 10.2 | 9.94 | ||
| S/N | 52 | 41 | 350 | 253 | 243 | 117 | ||
| LC–MS (MRM) | Accuracy (%) | 110 | 100 | 114 | 101 | 110 | 108 | |
| Precision (%) | 6.12 | 8.59 | 6.58 | 2.85 | 1.97 | 4.68 | ||
| S/N | 327 | 702 | 50 | 384 | 436 | 1637 | ||
|
LQC (80 ng/mL) |
LC-vDMS-MS (SIM) | Accuracy (%) | 100 | 95.4 | 93.4 | 99.3 | 106 | 86.1 |
| Precision (%) | 8.12 | 6.71 | 7.23 | 12.5 | 11.3 | 5.63 | ||
| S/N | 424 | 178 | 1492 | 694 | 622 | 367 | ||
| LC–MS (MRM) | Accuracy (%) | 93.3 | 89.3 | 87.4 | 87.0 | 89.8 | 92.0 | |
| Precision (%) | 5.94 | 4.84 | 4.28 | 9.45 | 7.13 | 3.67 | ||
| S/N | 594 | 1296 | 120 | 1096 | 2157 | 2343 | ||
|
MQC (50 ng/mL) |
LC-vDMS-MS (SIM) | Accuracy (%) | 98.5 | 93.9 | 94.8 | 93.3 | 93.6 | 98.4 |
| Precision (%) | 7.44 | 10.8 | 9.59 | 8.46 | 9.12 | 8.15 | ||
| S/N | 1964 | 448 | 6551 | 2135 | 2868 | 901 | ||
| LC–MS (MRM) | Accuracy (%) | 99.5 | 108 | 92.3 | 96.6 | 111 | 104 | |
| Precision (%) | 7.80 | 6.43 | 4.29 | 5.66 | 5.85 | 10.9 | ||
| S/N | 8018 | 12,012 | 402 | 8805 | 24,475 | 17,128 | ||
|
HQC (1500 ng/mL) |
LC-vDMS-MS (SIM) | Accuracy (%) | 101 | 94.1 | 93.7 | 96.5 | 95.3 | 92.0 |
| Precision (%) | 11.4 | 9.57 | 7.02 | 6.35 | 9.17 | 9.21 | ||
| S/N | 2797 | 898 | 6702 | 3585 | 3946 | 1499 | ||
| LC–MS (MRM) | Accuracy (%) | 91.5 | 97.3 | 93.8 | 94.9 | 93.4 | 95.0 | |
| Precision (%) | 9.29 | 6.15 | 9.29 | 7.33 | 4.94 | 6.39 | ||
| S/N | 17,696 | 18,303 | 860 | 17,219 | 41,603 | 34,722 |