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. 2022 Jan 19;414(25):7461–7472. doi: 10.1007/s00216-022-03881-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Comparison of different classifiers for the determination of the menstrual cycle phase. A Progesterone levels in serum. B Progesterone levels in PF. C Combined serum classifier: (20αOHP4)/(CYP17A1 + HSD3B2); (20αOHP4: 20α-hydroxyprogesterone). D Combined PF classifier: (5αDHP4 + 20αOHP4)/CYP17A1. E Combined serum + PF classifier: (serum 5αDHP4 + serum 20αOHP4 + PF 20αOHP4)/PF CYP17A1; (5αDHP4: 5α-dihydroprogesterone). CYP17A1: 17OHP4/P4. HSD3B2: P4/P5. Ns, not significant. ***p-value < 0.001. ****p-value < 0.0001