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. 2022 May 5;3(5):100330. doi: 10.1016/j.xplc.2022.100330

Figure 2.

Figure 2

Cytogenetic analysis of Vanilla planifolia CR0040.

(A–D) Orcein staining: (A and B) mitotic metaphases with 2n = 32 chromosomes; (C) karyotype corresponding to (B); (D) hypoaneuploid mitotic metaphase with 2n = 28 chromosomes; (E) karyotype corresponding to (D); (F) interphase nuclei showing heterochromatic chromocenters; (G) DAPI-stained interphase nucleus showing unspecific heterochromatin; (H) chromomycin fluorochrome staining with two CMA+ regions (arrows) corresponding to rDNA sites; (I) Hoechst-stained AT-rich DNA in metaphase and interphase nucleus (IN), with two fully heterochromatinized chromosomes (arrows). Scale bars represent 10 μm.