FIG. 2.
l-[14C]arabinose accumulation by E. coli CW2587 with various transporter-reporter systems. Cells harbored arabinose transport genes on pAK01 (PtaclacUV5::araE), pAK02 (Ptac::araE), or pKKATEB (Ptac::araFGH) and the reporter gene on pCSAK50 (araC-PBAD::gfpuv). Data for cultures preinduced with 32 μg of IPTG per ml for 6 h prior to addition of l-[14C]arabinose (black bars) and uninduced cells (open bars) are shown. Assays were performed 2 min after radioactive arabinose was added. All numbers are the averages of triplicate experiments.