TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and characteristicsa | Reference or source |
|---|---|---|
| E. coli strains | ||
| CW2513 | K-12 wild type | 5 |
| CW2549 | araE201 | 5 |
| CW2553 | araE201 ΔaraFGH::kan | 5 |
| CW2587 | araE201 ΔaraFGH::kan srl::Tn10 recA59 | 5 |
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu) 7697 galU galK1 rpsL nupG | Life Technologies Inc. (Gaithersburg, Md.) |
| Plasmids | ||
| pAK01 | pMMB206 lacIqPtaclacUV5::araE Cmr | This work |
| pAK02 | pMMB207 lacIqPtac::araE Cmr | This work |
| pCSAK50 | pTC40 PBAD::gfpuv Apr | This work |
| pGFPuv | pUC18 gfpuv Apr | Clontech |
| pKKATEB | pKK223-3 Ptac::araFGH Kanr Apr | 5 |
| pTC40 | pBAD24 PBAD::lacZ Apr | 11 |
a
Cmr, chloramphenicol resistance; Apr, ampicillin resistance; Kanr, kanamycin resistance.