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Effect of L2HGDH shRNA on expression of HNF1α and HNF1α. Primary RPT cells were transduced with lentiviral L2HGDH shRNA or Control TRC shRNA. A portion of the cultures was transferred either into matrigel or onto plastic. The relative level of mRNA for (A) HNF1α and (B) HNF1β was determined as described in Materials and Methods. (*) p < 0.05 relative to Con TRC Monolayers; (#) p < 0.05 relative to Con TRC Matrigel cultures.
