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. 2022 Sep 5;13:932286. doi: 10.3389/fendo.2022.932286

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Copyright © 2022 Taub, Mahmoudzadeh, Tennessen and Sudarshan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of L2HGHD shRNA and siRNA on DNA methylation. The effect of L 2HGDH shRNA and siRNA on 5hmC levels. Genomic DNA was purified from primary cultures (A) transduced with lentivirus containing either Scrambled shRNA or L2HGDH shRNA vectors, or (B) transfected with either Negative Control siRNA, or Silencer Select L2HGDH siRNA. Serial dilutions of the genomic DNA were applied to slot blots. Subsequently, blots were probed with a 5hmC antibody, and total DNA visualized with methylene blue.