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Effect of L2HGDH siRNA on histone methylation. Nuclear histones were purified from primary cultures transfected either with (A) L2HGDH Stealth siRNA or Scrambled Control Stealth siRNA, or (B) Silencer Select L2HGDH siRNA or Negative Control siRNA. Three days later, nuclear histones were purified. Subsequently, nuclear histones were separated by SDS/PAGE, transferred to nitrocellulose, followed by Western analysis, as described in Materials and Methods.
