Figure 7.

Effect of L2HGDH shRNA and L2HGDH siRNA on the expression of transporter mRNAs in monolayer cultures. (A) The expression of the mRNAs for L2HGDH, NPT2a, OAT1, GAPDH and SGLT2 was determined in primary cultures transduced with lentivirus containing either an L2HGDH shRNA or Control TRC vector. The relative expression of mRNAs in cultures transduced with L2HGDH shRNA was compared to the level in cultures transduced with Control TRC shRNA. (B) Relative mRNA levels were determined in primary RPT cells transfected twice with either L2HGDH Stealth siRNA or Scrambled siRNA. (C) Relative mRNA levels were determined in primary RPT cells transfected twice with either L2HGDH Silencer Select siRNA or Negative Control siRNA, as described in Materials and Methods. In part A, (*) p < 0.05 relative to either the TRC Control (Part A), the Scrambled Control (Part B), or, the Negative Control (Part C).