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Effect of matrigel on transporter gene expression. Primary cultures of RPT cells were transduced with lentiviral L2HGDH shRNA or Control TRC shRNA. A portion of the cultures was transferred into either matrigel or onto plastic. The relative level of mRNA for (A) SGLT2, (B) NPT2a, and (C) AQP1 was determined as described in Materials and Methods. (*) p < 0.05 for the Con TRC Plastic Control; (**) p < 0.05 for the ConTRC Matrigel Control.
