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(A,B) Cytotoxicity and IC50 (µg/ml) values of AP-ZEONE against T47D, MCF-7, MDA. MB.231, and L929 cell lines. The treatment times were 24, 48, and 72 h, respectively. Apoptotic cells were observed (C) as rounded and non-adherent entities, (D) blebbing and DNA fragmentation (E,F) phosphatidylserine externalization at AP-ZEONE IC50 compared to control intact cells. The “*** “, “** “and “* “indicate the level of statistical significance p < 0.001, p < 0.01 and p < 0.05, respectively.
