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. 2000 Dec;182(24):7035–7043. doi: 10.1128/jb.182.24.7035-7043.2000

FIG. 1.

FIG. 1

Expression of moa: ModE-dependent molybdate and tungstate regulation. All growths were performed at 37°C in LB medium. Additions to the growth media were sodium molybdate and sodium tungstate to a final concentration of 10 mM, as indicated. β-Galactosidase activity is expressed in Miller units (24). (A) Strains defective in molybdenum cofactor (moa), under anaerobic growth. (B) Strains which were molybdenum cofactor sufficient, under anaerobic growth. (C) Strains defective in molybdenum cofactor (moa), under aerobic growth. (D) Strains which were molybdenum cofactor sufficient, under aerobic growth. The strains used were as follows: 1 and 7, DB1004 [φ(moaB::lacZ)4]; 2 and 8, LA27 (as DB1004; modC118); 3 and 9, LA29 (as LA27; modE::Gm); 4 and 10, DB1060 [λpφ(moaB::lacZ)4]; 5 and 11, TL28 (as DB1060; modC118); 6 and 12, LA30 (as TL28; modE::Gm).