TABLE 1.
Bacterial strains, bacteriophage, and plasmids
| Strain, bacteriophage, or plasmida | Relevant genotype or characteristics | Source or reference |
|---|---|---|
| Strains | ||
| RK4353 | F′ araD139 Δ(argF-lac)U169 deoC1 flbB5201 gyrA 219 relA1 rpsL150 non-9 ptsF25 | 37 |
| DB1004 | As RK4353; φ(moaB::lacZ)4 | 3 |
| DB1060 | As RK4353; [λpφ(moaB::lacZ)4] | 3 |
| SE1597 | Δ(lacU)169 rpsL; modC118 | K. T. Shanmugan |
| RK4921 | As RK4353; zbh-623::Tn10 (near mod) | V. Stewart |
| LA27 | As DB1004; modC118 zbh-623::Tn10 | This work |
| LA29 | As LA27; modE::Gm | This work |
| TL28 | As DB1060; modC118 zbh-623::Tn10 | This work |
| LA30 | As TL28; modE::Gm | This work |
| MC4100 | F′ araD139 Δ(argF-lac)U169 deoC1 flbB5201 relA1 rpsL150 ptsF25 rbsR | Lab stocks |
| RM102 | As MC4100; fnr Δ(srl-recA)306::Tn10 | A. Bock |
| MC1000 | F′ araD139 Δ(araABC-leu) 7679 galU galK Δ(lac)X74 rpsL thi | Lab stocks |
| HORF55 | As MC1000; modE::Gm | 38 |
| NS3 | As MC4100; moeB | Lab stocks |
| Bacteriophage | ||
| λpφ(moa-lacZ)4 | λpφ(moaB::lacZ)4 | This work |
| Plasmids | ||
| pMLB524 | Ampr [Lac+] | 35 |
| pAA182 | Ampr ′lacZ | 14 |
| pEM101 | 1.16-kb PvuII fragment of moa DNA in pAA182 | This work |
| pEM114* | As pEM101, but 0.74-kb insert | This work |
| pEM117* | As pEM101, but 0.80-kb insert | This work |
| pEM118* | As pEM101, but 0.54-kb insert | This work |
| pEM220† | As pEM101, but 0.37-kb insert | This work |
a
*, insert constructed by deleting from the pAA182 multiple cloning site an EcoRI site upstream of the insert in plasmid pEM101; †, constructed by deleting from the vector's multiple cloning (BamHI) site downstream of the insert in pEM101.