Figure 3.

Drought tolerance of CSSL29, functional verification of Goano05G0268 by VIGS in CSSL29, and ectopic expression in Arabidopsis.

(A) Phenotypic comparison of Su8289 and G. anomalum in response to water deficit. Scale bar, 5 cm.

(B) Phenotypic comparison of Su8289 and CSSL29 in response to drought stress using 20% PEG treatment. Photographs were taken at 0 and 72 h after PEG treatment. Scale bar, 5 cm.

(C) Expression level of Goano05G0268 in Su8289 and CSSL29 under PEG stress at 0, 6, 12, 24, and 72 h. The left y axis shows the relative expression according to qRT–PCR, and the right y axis shows the FPKM (fragments per kilobase per million mapped reads) value obtained from RNA-seq data. ∗∗P < 0.01, Student’s t-test.

(D) Transcript levels of Goano05G0268 in leaves from CSSL29 plants infected with pTRV2 and pTRV2-Goano05G0268 under PEG stress at 72 h, evaluated by qRT–PCR. ∗∗P < 0.01, Student’s t-test.

(E) Phenotypes of CSSL29 plants infected with pTRV2, pTRV2-Goano05G0268, and pTRV2-CLA. Scale bar, 5 cm.

(F) Phenotypic comparison of Arabidopsis EE and WT seedlings after 25 days of drought stress.

(G) Endogenous H₂O₂ content, MDA content, and POD activity of Arabidopsis EE and WT lines before and after 14 days of drought stress. ∗∗P < 0.01, Student’s t-test.