CT glycan antigen expression by WFA-FITC labeling of GalNAc in biopsy sections
(A) The percentage of GalNAc-positive fibers for both subjects across time points is shown on the left, and the same data are represented as a fold change versus baseline (horizontal dashed line) for each subject on the right. Data for fibers from all blocks representing a given time point was pooled to produce a single value for each time point. (B) GalNAc intensity in all fibers normalized to the healthy control median intensity (horizontal dashed line) shown for each time point on the left, and for only GalNAc-positive muscle fibers on the right. Each point represents a single muscle fiber, and the solid horizontal line at each time point represents the mean of all muscle fibers. (C) WFA-FITC fluorescence (green) labeling GalNAc in representative muscle regions counter-stained for spectrin (magenta) at baseline and the first post-treatment time point for each subject, compared with a representative healthy control sample. Fiber GalNAc positivity and normalized fiber GalNAc intensity analysis results are shown in the third and fourth columns using color-coded heatmaps. Color keys indicating the conversion scale for percentage GalNAc-positive perimeter and fold GalNAc intensity (normalized to healthy control median) are shown below each column. Gray color indicates regions of high connective tissue accumulation that were masked to avoid interference with the automated analysis. All regions are 2 × 3 mm.