Figure 4.

Regional variability of GalNAc expression at the first post-treatment time point

(A) GalNAc signal quantification for high- and low-expressing regions separated along the fascia in whole-muscle sections from the first treated time point. On the right, each point represents a single muscle fiber and the solid horizontal line at each time point represents the mean of all muscle fibers. The horizontal dashed line represents the healthy control median GalNAc intensity. (B) Images of tissue sections from subjects 1 and 2 showing variable regional GalNAc expression (green), with high-expressing fiber regions outlined in red and low-expressing regions outlined in cyan. Fiber GalNAc positivity analysis results are shown in the lower row of images using color-coded heatmaps. The color key indicating the conversion scale for percentage GalNAc-positive perimeter is shown below the images. Scale bar, 1mm.