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. 2022 Sep 13;3(4):101668. doi: 10.1016/j.xpro.2022.101668
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

Premium plus fetal bovine serum Thermo Fisher Scientific Cat#A4766
RPMI 1640 with L-Glutamine Sigma-Aldrich Cat#R1383
Penicillin-Streptomycin Sigma-Aldrich Cat#P0781
D- (+) Glucose Sigma-Aldrich Cat#G8270
Phosphate buffer saline (PBS) Sigma-Aldrich Cat#D8537
H2O HPLC grade Sigma-Aldrich Cat#270733
Acetonitrile LC-MS grade Sigma-Aldrich Cat#1000291000
D-Glucose (1,2-13C2, 99%) Cambridge Isotope Laboratories Cat#CLM-504-PK
RPMI 1640 Medium, no glucose Thermo Fisher Scientific Cat#11879020
Red Blood Cell Lysis Buffer Abcam Cat#Ab204733
TLR4 Agonist - Ultrapure LPS from E. coli 055:B5 InvivoGen Cat# tlrl-pb5lps
Pierce™ BCA Protein Assay Kit Thermo Fisher Scientific Cat#23225

Deposited data

Metabolomics Data MassIVE MSV000088899

Experimental models: Organisms/strains

C57BL/6J (for peritoneal isolation) male & female mice, 8–12 weeks old, wild type NA NA

Software and algorithms

MetaboAnalyst 4.0 (Chong et al., 2019) https://www.metaboanalyst.ca/
Xcalibur 4.0 software Thermo Fisher Scientific
Tracefinder 2.1 software Thermo Fisher Scientific

Other

Surgical scissors NA NA
Forceps NA NA
Hemostat Forceps, flat NA NA
Cell culture dishes, 24 well Thermo Fisher Scientific Cat#142475
Falcon 50 mL Conical Centrifuge Tubes Fisher Scientific Cat#352070
Needles, 25G & 27G Fisher Scientific Cat#305127
BD Syringe with Luer-Lok Tips (Without Needle), 10 mL Fisher Scientific Cat#301029
70% Ethanol NA NA
Ice Bucket NA NA
1.5 mL Microcentrifuge Tubes Fisher Scientific Cat#3448PK
Thermo Q-Exactive Thermo Fisher Scientific Cat# IQLAAEGAAPFALGMBDK
Ultimate3000 series HPLC Thermo Fisher Scientific Cat#4832.5000A
Xbridge Amide column (4.6 mm × 100 mm and a 3.5 μm particle size) Waters Cat#186006595