| Chemicals, peptides, and recombinant proteins |
|
| Premium plus fetal bovine serum |
Thermo Fisher Scientific |
Cat#A4766 |
| RPMI 1640 with L-Glutamine |
Sigma-Aldrich |
Cat#R1383 |
| Penicillin-Streptomycin |
Sigma-Aldrich |
Cat#P0781 |
| D- (+) Glucose |
Sigma-Aldrich |
Cat#G8270 |
| Phosphate buffer saline (PBS) |
Sigma-Aldrich |
Cat#D8537 |
| H2O HPLC grade |
Sigma-Aldrich |
Cat#270733 |
| Acetonitrile LC-MS grade |
Sigma-Aldrich |
Cat#1000291000 |
| D-Glucose (1,2-13C2, 99%) |
Cambridge Isotope Laboratories |
Cat#CLM-504-PK |
| RPMI 1640 Medium, no glucose |
Thermo Fisher Scientific |
Cat#11879020 |
| Red Blood Cell Lysis Buffer |
Abcam |
Cat#Ab204733
|
| TLR4 Agonist - Ultrapure LPS from E. coli 055:B5 |
InvivoGen |
Cat# tlrl-pb5lps |
| Pierce™ BCA Protein Assay Kit |
Thermo Fisher Scientific |
Cat#23225 |
|
| Deposited data |
|
| Metabolomics Data |
MassIVE |
MSV000088899 |
|
| Experimental models: Organisms/strains |
|
| C57BL/6J (for peritoneal isolation) male & female mice, 8–12 weeks old, wild type |
NA |
NA |
|
| Software and algorithms |
|
| MetaboAnalyst 4.0 |
(Chong et al., 2019) |
https://www.metaboanalyst.ca/ |
| Xcalibur 4.0 software |
Thermo Fisher Scientific |
|
| Tracefinder 2.1 software |
Thermo Fisher Scientific |
|
|
| Other |
|
| Surgical scissors |
NA |
NA |
| Forceps |
NA |
NA |
| Hemostat Forceps, flat |
NA |
NA |
| Cell culture dishes, 24 well |
Thermo Fisher Scientific |
Cat#142475 |
| Falcon 50 mL Conical Centrifuge Tubes |
Fisher Scientific |
Cat#352070 |
| Needles, 25G & 27G |
Fisher Scientific |
Cat#305127 |
| BD Syringe with Luer-Lok Tips (Without Needle), 10 mL |
Fisher Scientific |
Cat#301029 |
| 70% Ethanol |
NA |
NA |
| Ice Bucket |
NA |
NA |
| 1.5 mL Microcentrifuge Tubes |
Fisher Scientific |
Cat#3448PK |
| Thermo Q-Exactive |
Thermo Fisher Scientific |
Cat# IQLAAEGAAPFALGMBDK |
| Ultimate3000 series HPLC |
Thermo Fisher Scientific |
Cat#4832.5000A |
| Xbridge Amide column (4.6 mm × 100 mm and a 3.5 μm particle size) |
Waters |
Cat#186006595 |