Fig. 1.

In vitro NETs formation. (A) NETs structures stained by SYTOX Green with and without DNase I and DPI stimulation in different sample extractions (scale bar = 50 μm); PMA (100 nM) was used as a positive control. (B) Quantitative determination of dsDNA in the supernatant of neutrophils, and (C) MPO-DNA complexes levels quantified by ELISA with and without DNase I and DPI stimulation (**: p < 0.01; ***: p < 0.001). (D) Immunofluorescent images of CitH3 (NETs marker, red) expression in neutrophils cultured in different groups (scale bar = 50 μm).