TABLE 3.
MarA activation at class II promoters with alanine substitutions in α-NTD
| α-NTD derivative | β-Galactosidase sp act (% of wild-type activity)a
|
||
|---|---|---|---|
| Φ(inaA-lacZ) | Φ(fumC-lacZ) | Φ(micF-lacZ) | |
| Wild type | 88/107 (100) | 326/376 (100) | 702/559 (100) |
| 162A | 87/110 (101) | 338/309 (92) | 648/556 (95) |
| 163A | 88/107 (100) | 339/373 (101) | 719/504 (97) |
| 164A | 95/113 (107) | 303/394 (99) | 686/581 (100) |
| 165A | 126/115 (124) | 317/394 (101) | 659/586 (99) |
| 162–165A | 102/113 (110) | 312/436 (107) | 669/529 (95) |
a
β-Galactosidase specific activity (in Miller units) was measured from single- copy inaA-, fumC- and micF-lacZ fusions in strains N8457, N9638 and N9639 (21), respectively, transformed with plasmids encoding either wild-type or substitution derivatives within the N-terminal domain of α. Cultures were grown in the presence of the inducer salicylate. Data from experiment 1 are shown before the slash, and data from experiment 2 are shown after the slash.