TABLE 4.

SoxS activation at class II promoters ith alanine substitutions in α-NTD

Promoter	rpoA mutation	Activity (Miller units)a		Induction ratio	% of wild-type activity
		Uninduced	Induced
fumC	Wild type	66	2210	33	100
	162A	72	2540	35	115
	163A	74	2120	29	96
	164A	71	2000	28	90
	165A	74	2400	32	109
	162–165A	82	2920	36	132
micF	Wild type	43	325	7.5	100
	162A	44	390	8.8	120
	163A	45	335	7.4	104
	164A	45	400	8.9	123
	165A	47	290	6.2	89
	162–165A	63	345	5.5	107
nfo	Wild type	225	2400	10.6	100
	162A	205	2000	9.7	84
	163A	225	2070	9.2	86
	164A	215	2100	9.7	88
	165A	205	2460	12.1	103
	162–165A	205	2440	12.0	102
sodA	Wild type	1,080	9,530	8.8	100
	162A	915	8,990	9.8	94
	163A	1,105	8,770	7.9	92
	164A	905	9,560	10.6	100
	165A	880	7,930	9.0	83
	162–165A	805	8,960	11.2	94

^aβ-Galactosidase specific activity was measured from single-copy lacZ fusions in a wild-type strain background transformed with plasmids encoding either wild-type or substitution derivatives within the N-terminal domain of α. Cultures were grown either in the absence or the presence of the inducer paraquat. The percent wild-type value was calculated from the induced Miller unit value in the presence of the α-NTD mutant compared with the induced Miller unit value obtained with wild-type α. Values are the averages of three independent experiments.