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. 2022 Sep 14;21:381–393. doi: 10.1016/j.bioactmat.2022.08.022

Fig. 5.

Fig. 5

TWNDs maintained the function of mitochondria and increased mitophagy. (A–C) TEM image of tubular cells in sham mouse (A), AKI mouse (B), AKI mouse after injection of TWNDs (C). Scale bar: 2 μm. (D) Schematic illustration of TWNDs restoring mitochondrial autophagy. (E) WB analysis of PARKIN, PINK1, p62/SQSTM1, and LC3BII/I proteins expression in renal tissue homogenate from each group. (F–I) Quantification of the protein immunoblots of PARKIN (F), PINK1 (G), p62/SQSTM1 (H), LC3BII/I (I). (J) Immunofluorescent staining of JC-1 in HK-2 cells from each group. Scale bar: 20 μm. (K) Quantification of mitochondrial membrane potential (MMP) of HK-2 cells under different treatments by Flow Cytometry. (L) ATP production rate of HK-2 cells under different treatments. Data represent means ± S.D. from at least three independent replicates. (*P < 0.05, **P < 0.01, ***P < 0.001 vs AKI or model groups; #P < 0.05, ##P < 0.01 vs sham or control groups).