. 2022 Sep 2;13:972041. doi: 10.3389/fphys.2022.972041

TABLE 4.

Effect of in ovo cysteine and lysine on serum biochemical indices of day-old chicks.

Parameters	UI	DW	L Y	CY	CLH	CLL	SEM	p Value
Total protein (mg/dl)	8.07^c	8.20^c	8.83^b	8.76^b	9.03^b	9.76^a	0.137	0.0001
Creatinine (mg/dl)	0.70	0.73	0.76	0.76	0.73	0.73	0.50	0.647
ALT (U/L)	37.67	38.67	40.67	41.00	40.00	40.00	2.05	0.366
AST (U/L)	219.67	246.00	241.00	249.33	251.00	258.67	4.399	0.1509
Albumin (mg/dl)	3.26^ab	3.37^ab	3.20^b	3.23^ab	3.50^a	3.27^ab	0.031	0.0362
Globulin (mg/dl)	4.80^c	4.83^c	5.63^b	5.53^b	5.53^b	6.50^a	0.142	0.0001
ALB: GLB	0.68^a	0.70^a	0.57^bc	0.58^bc	0.63^ab	0.50^c	0.018	0.0002
Glucose (mg/dl)	324.33	363.33	359.67	366.33	361.67	350.00	20.33	0.081
Cholesterol (mg/dl)	208.00	234.67	237.33	241.33	240.00	243.67	16.84	0.065
Triglyceride (mg/dl)	91.00	95.33	94.67	96.00	93.33	86.33	8.03	0.754

^abc: Means value within the row bearing different superscript differ significantly (p< 0.05). UI, Un-injected eggs; DW, eggs injected with distilled water; LY, Eggs injected 2 mg lysine; CY, Eggs injected 3.4 mg cysteine; CLH, Eggs injected mixture of 3.4 mg cysteine plus 2 mg lysine; CLL, Eggs injected mixture of 1.7 mg cysteine plus 1 mg lysine.