Table 1. Analytical Data of the Synthetic Peptides.
| peptide | sequence | UHPLC Rt (min) | ESI-MS(m/z)found (calcd) | HPLC purity % | quantity (mg) | yield (%) |
|---|---|---|---|---|---|---|
| hCAP(134–145) (A1) | H-LLGDFFRKSKEK-NH2 | 4.033a | 1467.9 (1466.8) [M + H]+ | 94.0% | 44 | 30% |
| hCAP(140–151) (A2) | Ac-RKSKEKIGKEFK-NH2 | 2.800a | 1518.9 (1518.9) [M + H]+ | 90.2% | 15 | 10% |
| hCAP(146–157) (A3) | Ac-IGKEFKRIVQRI-NH2 | 4.773a | 1528.0 (1527.9) [M + H]+ | 93.3% | 38 | 26% |
| hCAP(152–163) (A4) | Ac-RIVQRIKDFLRN-NH2 | 5.013a | 1599.0 (1598.9) [M + H]+ | 97.7% | 37. | 23% |
| hCAP(159–170) (A5) | Ac-DFLRNLVPRTES–OH | 4.720a | 1488.8 (1488.0) [M + H]+ | 99.3% | 43 | 29% |
a
Analytical RP-HPLC gradient: 10–60% B in 5 min.