Figure 4.

(A) Genome locality of ylbN and ylbN/0527 in B. subtilis and JCVI-syn3A, respectively. The ylbN genes are colored in red, while adjacent genes located prior to the dcw cluster in both organisms include rpmF, mraZ, and rsmH (also known as mraW) (blue), with additional genes only present in B. subtilis shown (green). Both gene clusters show similar orientation and arrangement on the reverse strand. (B) Structural alignment of AlphaFold2 prediction of ylbN/0527 (blue) and the predicted AlphaFold2 structure for ylbN in B. subtilis 168 (yellow⁷⁶). (C) CLUSTALW sequence alignment of ylbN/0527 and ylbN from B. subtilis with BLOSUM50 similarity coloring. The conserved 158R reported in ref (75) is aligned in the “DPR” region of both sequences.