Table 2.

Comparison of 3-hydroxyacyl-CoA dehydrogenase activity of human 17β-HSD10, 6xHis-tagged HSD10 and ABAD/ERAB

Substrate and coenzyme	17β-HSD10*		6xHis-tagged HSD10†		ABAD/ERAB¶
	kcat (s–1)	Km (μM)	kcat (s–1)	Km (μM)	kcat (s–1)	Km (μM)
NADH		20±2.8		89±13		ND
S-Acetoacetyl-CoA	37±1.6	89±5.4	74±6.8	134±14	190±19.8	68±20

^†Results of the present study by use of the experimental procedure a. No kinetic constants can be determined by use of the experimental procedure b same as that reported in the ABAD/ERAB study [35]; ^*Data from [3]; ND, Not determined; ^¶Data from [35].