FIG. 3.
Increased heat shock induction of ClpB1-79 protein in ANB1-79a strain. Wild-type Synechococcus (wt), ΔclpB1, ANB1-93, and ANB1-79a cultures were shifted from 37 to 48.5°C for 90 min. (A and B) Immunoblot detection (A) and quantification (B) of ClpB1-93 and -79 proteins. Levels of ClpB1-93 (●) and ClpB1-79 (○) in wild-type Synechococcus, ClpB1-93 (■) in ANB1-93, and ClpB1-79 (▴) in ANB1-79a were plotted relative to the 37°C control value for ClpB1-93 protein in wild-type Synechococcus, which was set at 1. (A) The figure shows a representative result from one of three independent replicates. (B) All values represent averages ± standard errors (n = 3).