Fig. 9.

NBTXR3 + XRT + PLT (Combo) induces immunological memory and upregulates immune activities in the blood. A Percentages of CD4⁺/CD45⁺ and CD8⁺/CD45⁺ T cells and memory CD4⁺ and CD8⁺ T cells in the blood and spleen. B Nanostring activity score of immune pathways and log₂ fold change in expression of genes involved in the adaptive pathway, NK cell function, B cell function, and T cell function with the control as the baseline. The five mice that survived in the NBTXR3 + XRT + PLT group in Fig. 1 were re-challenged with 5 × 10⁴ 344SQR cells on the right flank at least 60 d after the last fraction of 12 Gy. Five mice with 5 × 10⁴ 344SQR cells inoculated on the right flank served as the control. All the mice were euthanized 28 d after tumor inoculation. Blood was harvested before euthanizing the mice, red blood cells were lysed, and RNA was extracted for Nanostring analysis. The populations of memory CD4⁺ and CD8⁺ T cells were analyzed by flow cytometry. P < 0.05 was considered statistically significant. *P < 0.05, **P < 0.01, ***P < 0.001, NS, not significant