Figure 1.

Accumulation of anti-SARS-CoV-2 nAbs in agroinfiltrated FX-KO N. benthamiana plants. Western blot analysis of extracts of N. benthamiana plants syringe co-agroinfiltrated with A. tumefaciens strains harboring the HC and LC of either mAb675 (A) or mAbJ08-MUT (B). Samples obtained from leaves (taken at 2, 3, 6, or 7 d.p.i.) were separated on reducing (R) or non-reducing (NR) 12% SDS-PAGE. Plant extracts were normalized for total soluble protein (TSP) content; 10 μg of TSP was loaded for each sample. Extracts from plants infiltrated with only P19 silencing suppressor were used as negative control (C-). As positive control (C+), 50 ng of purified human IgG1 mAbRTX was used. Detection was performed with an anti-human γ chain (HC) or anti-human κ chain (LC) HRP-conjugated antibody.