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. 2022 Sep 5;13:956741. doi: 10.3389/fpls.2022.956741

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Copyright © 2022 Frigerio, Marusic, Villani, Lico, Capodicasa, Andreano, Paciello, Rappuoli, Salzano, Scaloni, Baschieri and Donini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of LC expression levels on the accumulation of antibodies. Western blot analysis of leaves agroinfiltrated with single LCs of mAbJ08-MUT and mAb675 or with HC and LC combinations of the two nAbs (J08-MUT-HC-675 LC and 675 HC-J08-MUT LC). Agroinfiltrated leaves were collected on day 6 post-infiltration. Extracts normalized for total soluble protein (TSP) content were separated on non-reducing (NR) 12% SDS-PAGE; 15 μg of TSP was loaded for assembled antibody samples, while 45 μg of TSP was loaded for the two 675 LC and J08-MUT-LC samples. C+: 50 ng of purified human IgG1 mAbRTX. Detection was performed with an anti-human γ chain (HC) or anti-human κ chain (LC) HRP-conjugated antibody.