Figure 4.

Purification and characterization of the plant-derived antibodies. AntiSARS-CoV-2 nAbs were purified by protein-A affinity chromatography and eluted fractions (FX-10 μL per lane) were separated by 12% SDS-PAGE under reducing (left side) and non-reducing conditions (right side), followed by Coomassie blue staining (A). FT: flow-through of protein-A column; C+: purified human IgG1 mAbRTX used as a reference (2 μg). (B) Size-exclusion chromatography was performed on a Superdex^™ 75 10/300 GL column, as described in the Section Materials and methods. The retention volumes (mL) of the peaks obtained and the schematic representation of the molecules contained in the eluted peaks are indicated.