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. 2021 Dec 22;71(10):2011–2021. doi: 10.1136/gutjnl-2020-323951

Figure 6.

Figure 6

Antitumour molecules produced from L. gallinarum are non-protein with a molecular weight <3 kDa. (A) LMW-LGCS but not HMW-LGCS significantly suppressed cell growth of HCT116 and LoVo. (B) Decrease in proliferation of CRC cells was observed in heat-inactivated LGCS. (C) Decrease in proliferation of CRC cells was observed in PK-inactivated LGCS. (D) Score plots of PCA revealed clear separations of metabolites in culture supernatant of L. gallinarum, E. coli MG1655 and BHI groups. (E) Heatmap analysis revealed the abundance of different metabolites in LGCS, ECCS and BHI groups. (F) Score plots of PCA revealed clear separations among L. gallinarum, E. coli MG1655 and PBS-treated Apc Min/+ mice. (G) Heatmap analysis revealed the abundance of different metabolites in the gut of Apc Min/+ mice under different treatments. P values are calculated by two-way analysis of variance or Student’s t-test as appropriate. *P<0.05, **p<0.01, ****p<0.0001. BHI, brain heart infusion; CRC, colorectal cancer; ECCS, Escherichia coli culture supernatant; HMW, high molecular weight; LGCS, Lactobacillus gallinarum culture supernatant; LMW, low molecular weight; PBS, phosphate-buffered saline; PCA, principal component analysis; PK, proteinase K.