Table 7. Cumulus oophorus cells number, apoptotic cumulus oophorus cells and cumulus oophorus cells apoptotic index (LSM ±SEM) from mid-lactating Girolando dairy cows fed diets with different crude protein (CP) concentrations.
| Cumulus oophorus cells | Treatments (g Kg-1 DM) | P-value | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 156 (3) | 139 (19) | 132 (19) | 127 (34) | Linear | Quad | |||||
| Total number | 175.67 | ±102.06 | 308.30 | ±67.1 | 162.49 | ±38.58 | 589.10 | ±108.32 | 0.0238 | 0.3500 |
| TUNEL-positive | ||||||||||
| Number | 3.67 | ±4.05 | 28.46 | ±10.54 | 10.71 | ±4.35 | 1.39 | ±0.6 | 0.0500 | 0.0449 |
| Index | 0.08 | ±0.054 | 0.10 | ±0.026 | 0.14 | ±0.036 | 0.06 | ±0.03 | 0.5616 | 0.2333 |