TABLE 3.
Summary of purification of R. capsulatus DXPase-A
| Step | Amt of protein (mg) | Sp acta (μmol/min/ mg) | Total activity (μmol/min) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|
| Supernatant | 493 | 0.18 | 89 | 1 | 100 |
| 40% (NH4)2SO4 precipitate | 213 | 0.35 | 75 | 2 | 84 |
| Ni2+-silica affinity column | 25 | 1.6 | 40 | 10 | 45 |
a
DXPase-A activity was determined with 25 mM [2-14C]pyruvate (0.06 μCi/μmol), 100 mM d-glyceraldehyde, and 2 mM MgCl2. Reactions were quenched by the addition of EDTA to a final concentration of 250 mM as described in Materials and Methods.