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. 2022 Jul 4;81(10):1400–1408. doi: 10.1136/annrheumdis-2022-222229

Figure 3.

Figure 3

Neutrophil infiltration in mice with severe psoriasis-like disease. (A) S100A9 (red) and Ly6B (neutrophils; green) immunostaining of lesional ears in mice with inducible dual epidermal deletion of c-Jun and junB (DKO*) and triple epidermal deletion of c-Jun, JunB and S100A9 (TKO*) (dotted lines represent the basal membrane, yellow arrows point to Ly6B/S100A9-double positive cells, scale bar=20 µm). (B–C) Confocal microscopy–based quantification of absolute number of neutrophils (Ly6B-positive) (B) and S100A9-positive neutrophils (C) in the whole ear sections of DKO* and TKO* mice and wild-type (WT) littermates (n=4–5 mice). (D) S100A9 (red) and Ly6B (green) immunostaining of lesional psoriatic arthritis (PsA)–like paws (scale bar=200 µm). Yellow arrows point to infiltrating cells. (E–F) Confocal microscopy–based quantification of neutrophil (Ly6B-positive) (E) and S100A9-positive neutrophil (F) in the distal interphalangeal joints of DKO* and TKO* mice and WT littermates (average of 3–5 regions per paw, n=4 mice).