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. 2001 Jan;183(1):12–27. doi: 10.1128/JB.183.1.12-27.2001

TABLE 3.

Antibiotic resistance of ΔoprM P. aeruginosa K1110 expressing mutant OprM proteinsa

Strain Plasmid OprM derivative OprM expressionb MIC (μg/ml) ofc:
CAR CPZ CIP NOR CAM NOV
ML5087 Wild type + 32–64 8 0.2 1 64 128
K1110 − 0.5 1 0.1 0.5 64 16
K1110 pVLT31 − 1 1 0.1 0.5 64 16
pXZL34 Wild type ++ 64 8 0.2 1 128 128
pXZL74 ΔP5-P10 ++ 32 8 0.2 0.5 128 128
pXZL88 ΔT28-D37 ++ 64 8 0.1 1 64 128
pXZL92 ΔR74-I79 ++ 32 4 0.2 1 64 128
pXZL57 ΔS93-G107 + 64 4 0.1 1 64 64
pXZL137 ΔA12-R98 −de 8–16 1 0.1 0.5 64 16
pXZL58 T106-HA-T107 ++ 64 4 0.2 0.5 128 128
pXZL75 ΔE125-R131 − 0.5 1 0.1 0.5 32 16
pXZL89 ΔA145-A153 −e 1 0.5 0.05 0.125 32 8
pXZL90 ΔG199-A209 + 0.5 1 0.05 0.25 64 16
pXZL73 ΔA233-G241 +/− 64 8 0.2 1 64 128
pXZL133 ΔL257-L264 + 64 8 0.2 1 64 128
pXZL77 ΔA278-N286 +/− 0.5 0.5 0.05 0.25 32 16
pXZL91 ΔF317-F326 −e 0.5 0.5 0.2 0.5 64 16
pXZL93 ΔR341-I349 − 0.5 0.5 0.05 0.25 32 16
pXZL76 ΔA445-W450 −de 2 2 0.05 0.125 32 16
pXZL66 ΔL446-A468 −e 0.5 1 0.1 0.25 32 16
pXZL67 ΔA451-A468 + 16 2 0.05 0.5 64 64
pXZL146 C1G + 64 4 0.2 1 128 128
pXZL86 S2D ++ 64 8 0.2 1 64 128
pXZL147 OprF-OprM fusion + 64 4 0.2 1 128 128
pXZL109 E125K + 64 8 0.2 0.5 64 128
pXZL125 L128R + 32 8 0.2 1 64 64
pXZL104 F129A + 64 8 0.2 1 64 128
pXZL105 F129Y + 64 8 0.2 1 64 128
pXZL128 R131E + 64 8 0.2 1 64 128
pXZL111 E279D ++ 64 8 0.2 0.5 128 128
pXZL108 E279K + 64 8 0.2 1 128 128
pXZL126 A284F ++ 64 8 0.2 1 64 128
pXZL129 A284Y ++ 64 8 0.2 1 128 128
pXZL106 L446V + 64 8 0.2 1 64 128
pXZL110 L446Q + 64 8 0.2 1 64 128
pXZL112 G448T + 64 8 0.2 1 64 128
pXZL107 G448A + 64 8 0.2 1 64 128
pXZL127 W450Y ++ 64 8 0.2 1 64 128
a

The antibiotic susceptibility of the ΔoprM strain K1110 harboring plasmids encoding the indicated OprM derivatives was determined as described in Materials and Methods. Susceptibility of the wild type (with respect to MexAB-OprM) parent strain ML5087 is shown for comparison purposes. 

b

Relative expression (taken from Fig. 2) indicated as ++ (high), + (moderate), +/− (weak), or − (none). 

c

Values in italics represent MICs substantially below that provided by the wild-type OprM protein. CAR, carbenicillin; CPZ, cefoperazone; CIP, ciprofloxacin; NOR, norfloxacin; CAM, chloramphenicol; NOV, novobiocin. 

d

OprM expression was detectable in cells following induction with IPTG. 

e

Weakly expressed in E. coli DH5α.