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. 2022 Sep 5;12:989167. doi: 10.3389/fonc.2022.989167

Figure 1.

Figure 1

IVM suppressed CTSB-induced NETs formation, which showed no effects on melanoma cell viability. (A) Schematic depicting treatment of bone marrow neutrophils for screening of the compound suppressed NETs. (B) ELISA analysis of Sytox green in bone marrow neutrophils of NETs. (C) ELISA analysis of mitochondrial DNA in bone marrow neutrophils treated with DMSO or IVM (5 µM) for 2 h. (D, E) FACS (Fluorescence activated cell sorting) analysis of the DCFH-DA in bone marrow neutrophils treated with DMSO or IVM (5 µM) for 2 h. (F, G) CCK8 analysis of cell viability in B16F10 treated with IVM (5 µM) for indicated times (F) or indicated concentrations of IVM for 48 h. Data in all panels are representative of at least three [(C–G), n = 3, mean ± SD] independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance [Significance was examined with two-way ANOVA in (C); Student’s t-test (E) or two-tailed unpaired Student’s t-test (F, G)].