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. 2022 Sep 5;12:989167. doi: 10.3389/fonc.2022.989167

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Copyright © 2022 Zhang, Xu, Xu and Ye

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IVM significantly suppressed CTSB-dependent NETs formation. (A) Confocal analysis of Sytox Green (green), Cit-H3 (red) and MPO (purple) in bone marrow neutrophils from wide type mice and treated with recombinant CTSB, CTSB with IVM or PMA after 12 h. (B) Percentage of released mitochondrial DNA in (A) was quantified (n = 3 field). Scale bars, 50 µm. (C) Quantitative analysis of mitochondrial DNA in the cultured medium. (D) SEM (scan electron microscopy) and quantitative analysis (E) of extracellular mitochondrial DNA induced by CTSB in bone marrow neutrophils after treated with IVM. (F) Confocal analysis of the expression of CTSB (green) and Ly6G (red) in lung of B16F10 mice. Data in all panels are representative of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 [one-way ANOVA in (B), (C) and (E)].