Skip to main content
. 2022 Sep 7;20(9):e3001782. doi: 10.1371/journal.pbio.3001782

Fig 6. FOPNL and CEP90 allow distal appendage proteins recruitment in mammalian cells.

Fig 6

(A) Serum-starved RPE1 cells treated with control (ctrl), FOPNL, or CEP90 siRNA were fixed and stained for GT335 (magenta) and antibodies directed against CEP90, FOPNL, OFD1, or the distal appendage proteins CEP83, CEP89, and CEP164 (green). Scale bar = 5 μm. (B) Quantification of the fluorescence intensity of FOPNL, CEP90, OFD1, CEP83, CEP89, and CEP164 at the centrosome after control, FOPNL, and CEP90 depletion. All data are normalized base on control siRNA and are presented as average ± SEM. ****p < 0.001 (one-way ANOVA followed by Tukey’s post hoc test), n ≥ 50 centrosomes from 2/3 independent experiments. Source data can be found in S4 Data. AU, arbitrary units; siRNA, small interfering RNA.