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(A) Functional complementation of chvH mutant A6880. Cells were grown on MG/L plates containing 0.2 g of SDS per liter at 28°C and observed after 3 days. (B) Measurement of VirE2 expression. A. tumefaciens strains were grown in IM containing 200 μM AS at 28°C for 20 h. Cells were pelleted, and total crude extracts were subjected to immunoblotting analysis as described in Materials and Methods. Strains: 1, A6007; 2, A6880(pWT155); 3, A6880(pWT181); 4, A6880(pSP329Gm).
