TABLE 2.
Effect of chvH mutation on expression of lacZ and phoA translational fusions to vir genesa
| Fusion (plasmid) | β-Galactosidase expression (Miller units)
|
|||
|---|---|---|---|---|
|
chvH strain (A348)
|
chvH::TnphoA strain (A6880)
|
|||
| IM | IM + 100 μM AS | IM | IM + 100 μM AS | |
| virB-lacZ (pTC112) | ND | 1,357 ± 5 | ND | 285 ± 6 (80%) |
| virE-lacZ (pTC111) | 46.6 ± 1.0 | 3,208 ± 46 | 15.1 ± 1.0 (70%) | 519 ± 15 (85%) |
| virG-lacZ (pWT160) | 77.5 ± 1.5 | 1,536 ± 39 | 48.0 ± 2.4 (40%) | 360 ± 10 (75%) |
| virA-phoA (pSL59) | 6.9 ± 0.7 | 59 ± 1.5 (8.6) | 6.9 ± 0.2 | 42 ± 1.0 (6) |
a
Data are means ± standard errors of the mean for three samples. The percentages in parentheses represent the reduction in expression in the chvH mutant relative to the wild-type strain. The numbers in parentheses are the fold increases in induction upon adding AS. The units are Miller units. ND, not determined.