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. 2022 Sep 19;12:15668. doi: 10.1038/s41598-022-19886-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Expression and purification of recombinant spike subunit fragments of SARS-CoV-2 and SARS-CoV. (A) Structure features of the SARS-CoV-2 spike (S) protein and the flow chart of protein library construction were generated by Microsoft PowerPoint 2016. The listed domain boundaries are mostly defined according to NTD, N Terminal Domain; RBD, Receptor Binding Domain; FP, Fusion Peptide; HR1, Heptad Repeat 1; HR2, Heptad Repeat 2; TM, Transmembrane Domain; CT, Cytoplasmic Tail. (For visual clarity, the length of the boxes is not proportional to the real sequence length). (B) Amino acid location and molecular weight of the protein fragments. (C and D) Twelve protein fragments expressing plasmids of SARS-CoV-2 and SARS-CoV were constructed and verified by agarose gel electrophoresis with restriction enzymes BamH I and Hind III. M: DNA markers of known size. Lane1-6: COVID19-SF1-6 (C) and SARS-SF1-6 (D). (E and F) SDS-PAGE analysis of the protein fragments expression of SARS-CoV-2 and SARS-CoV, respectively. M: Prestained protein markers of known size. Lane1-6: COVID19-SF1-6 (E) and SARS-SF1-6 (F).