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. Author manuscript; available in PMC: 2022 Dec 10.
Published in final edited form as: Sci Immunol. 2022 Jun 10;7(72):eabn5917. doi: 10.1126/sciimmunol.abn5917

Figure 1. Thpok binds NuRD.

Figure 1.

(A) SDS-PAGE and colloidal Coomassie blue staining of streptavidin pull down from activated Thpokfl/fl Ox40-Cre+ Rosa26BirA+ CD4+ T cells retrovirally transduced to express Thpok-Bio or with the empty retroviral vector (pMRx, Ctrl). Molecular markers are shown on the left.

(B) Thpok-associated NuRD-related peptides identified by mass spectrometry.

(C) Immunoblot analysis of streptavidin pull down (left panel) or whole cell lysates (right panel) from activated CD4+ T cells obtained from Thpokfl/fl Ox40-Cre+ Rosa26BirA+ that had been retrovirally transduced to express Thpok-Bio or with empty pMRx as a control vector (−). EtBr indicates treatment of cell lysates with Ethidium Bromide. Protein blots were probed with antibodies as indicated.

(D) Immunoblot analysis of streptavidin pull down (left panel) or whole cell lysates (right panel) from activated CD4+ T cells obtained from Thpok+/+ or ThpokBio/Bio Rosa26BirA+. Protein blots were probed with antibodies as indicated. Data are representative of three independent experiments.