FIG. 2.

Disruption of SEC20 alleles. (A) Genomic structures of wild-type SEC20 and its disrupted derivative alleles. The Ura-blaster module (hisG-URA3-hisG) was used to disrupt SEC20 alleles partially (sec20-Δ2 and sec20-Δ10). In addition, the SEC20 coding region was placed under transcriptional control of PCK1p (PCK1p-SEC20) or MET3p (MET3p-SEC20). The SalI sites within the YMR285 gene and the disruption fragments which generate specific SalI fragments in Southern blottings of genomic DNA (B) are shown in boldface. Strains analyzed were CAI4 (lane 1), CA2 (lane 2), CA2d (lane 3), CA2d2m (lane 4), and CAr2d110 (lane 5). The probe used in the Southern blotting experiment (B) is indicated by asterisks (A). Relevant fragment sizes are indicated (B; see text).