TABLE 1.
Strains and plasmids used
| Strain or plasmid | Genotype or description | Reference |
|---|---|---|
| C. albicans strains | ||
| 3153 | Prototrophic | 45 |
| SC5314 | Prototrophic | 13 |
| CAI4 | ura3Δ::imm434/ura3Δ::imm434 | 13 |
| CA2 | As CAI4, but SEC20/sec20Δ::hisG-URA3-hisG | This work |
| CA10 | As CA2 | This work |
| CA2d | As CAI4, but SEC20/sec20Δ::hisG | This work |
| CA10a | As CA2d | This work |
| CAr2d110 | As CAI4, but sec20Δ::hisG/PCK1p-SEC20 | This work |
| CAr10a3 | As CAr2d110 | This work |
| CA2d1m | As CAI4, but sec20Δ::hisG/MET3p-SEC20 | This work |
| CA2d2m | As CA2d1m | This work |
| S. cerevisiae strain RSY275 | MATα sec20-1 ura3-52 his4-619 | 17 |
| S. cerevisiae transformation vectors | ||
| STM20 | URA3-marked 2 μm vector with TPI1p-SEC20 | 43 |
| pYW9 | URA3-marked CEN vector with PFK2p-SEC20 | This work |
| pYW10 | URA3-marked 2 μm vector with PFK2-SEC20 | This work |
| pYW11 | URA3-marked CEN vector with SEC20 | This work |
| C. albicans vectors | ||
| pBI-1 | PCK1 promoter in pRC2312 | 42 |
| pCaDis | URA3-marked vector with MET3 promoter | 5 |
| pYW7 | PCK1p-SEC20 fusion in pBI-1 | This work |
| pYW20 | MET3p-SEC20Δ fusion in pCaDis | This work |